务川黑牛STAT3基因启动子区SNPs与生长相关性状的关联分析

doi:10.11843/j.issn.0366-6964.2013.08.005

Abstract

Abstract:

The study aimed to screen the SNPs in the promoter region of STAT3 gene in cattle and analyze the effect of SNPs on function elements of promoter and growth traits. Two cattle breeds (Wuchuan Black cattle and Guizhou Holstein cow) that possessed significant difference in breeds property were selected to construct DNA pools, SNP sites were screened by direct sequencing subsequently. PCR-RFLP was used to be genotyping and a total of 114 Wuchuan Black cattle were used to study the associations of SNPs with 8 growth traits. Results showed that 7 new single nucleotide polymorphism (SNP) sites including G-449A, C-341A, A-322G, C-246A, G-240A, C-225T and A-103T, respectively were found in the 5' flanking region of STAT3 gene and then submitted the information of SNPs to NCBI to obtain accession numbers. Furthermore, bioinformatics tools were used to predict the core region of the promoter and CpG island. SNPs found in this study led to the disappearance of a large amount of transcription factors bingding sites and the appearance of new sites, it also showed that SNP mutation didn’t affect the range of CpG island dramaticlly by using various softwares. Association analysis demonstrated that A-322G had highly significant effects on body weight (P<0.01), A allele was the favorable alleles. Moreover, animals with the genotype AG had the highest mean body weight than those with GG and AA genotypes, which suggested that A-322G was an important SNP marker affecting the cattle growth performance and STAT3 gene could be applied in marker-assisted selection for improving growth performance in cattle. This work could lay the experimental foundation for the identification of function of STAT3 gene in cattle.

CLC Number:

S823
S813.3

YANG Yong-qiang, JIAO Ren-gang, GONG Yu, XIE Hai-qiang, HUI Yan-ting, ZHANG Yi-yu, XIAO Chao-neng, LIU Ruo-yu. Associations of Polymorphisms of Promoter Region of STAT3 Gene with Growth Traits in Wuchuan Black Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1205-1212.

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References

［1］CALO V, MIGLIAVACCA M, BAZAN V, et al. STAT proteins: from normal control of cellular events to tumorigenesis ［J］. J Cell Physiol, 2003, 197(2):157-168.

［2］LANNING N J, CARTER-SU C. Recent advances in growth hormone signaling ［J］. Rev Endocr Metab Disorders, 2006, 7(4):225-235.

［3］HERRINGTON J, SMIT L S, SCHWARTZ J, et al. The role of STAT proteins in growth hormone signaling ［J］. Oncogene, 2000, 19(21):2585-2597.

［4］BUETTNER R, MORA L B, JOVE R. Activated STAT signaling in human tumors provides novel molecular targets for therapeutic intervention ［J］. Clin Cancer Res, 2002, 8(4):945-954.

［5］HIRANO T, ISHIHARA K, HIBI M. Roles of STAT3 in mediating the cell growth, differentiation and survival signals relayed through the IL-6 family of cytokine receptors ［J］. Oncogene, 2000, 19(21):2548-2556.

［6］MOLENAAR A, WHEELER T, MCCRACKEN J, et al. The STAT3-encoding gene resides within the 40 kbp gap between the STAT5A-and STAT5B-encoding genes in cattle ［J］. Anim Genet, 2000, 31(5):339.

［7］MEYER L, BROCKMANN G, SCHWERIN M, et al. A highly polymorphic GT-repeat in the bovine STAT5B encoding gene on BTA19 ［J］. Anim Genet, 1999, 30(1):72-73.

［8］BILD A H, TURKSON J, JOVE R. Cytoplasmic transport of Stat3 by receptor-mediated endocytosis ［J］. The EMBO J, 2002, 21(13):3255-3263.

［9］XU D, QU C K. Protein tyrosine phosphatases in the JAK/STAT pathway ［J］. Front Biosci J Virt Lib, 2008, 13: 4925-4932.

［10］CROKER B A, KIU H, NICHOLSON S E. SOCS regulation of the JAK/STAT signalling pathway ［J］. Semi Cell Dev Biol, 2008, 19: 414-422.

［11］SHUAI K. Regulation of cytokine signaling pathways by PIAS proteins ［J］. Cell Res, 2006, 16(2):196-202.

［12］AKIRA S. IL-6-regulated transcription factors ［J］. Int J Biochem Cell Biol, 1997, 29(12):1401-1418.

［13］WJST M, LICHTNER P, MEITINGER T, et al. STAT3 single-nucleotide polymorphisms and STAT3 mutations associated with hyper-IgE syndrome are not responsible for increased serum IgE serum levels in asthma families ［J］. Eu J Hum Genet, 2008, 17(3):352-356.

［14］HOLLAND S M, DELEO F R, ELLOUMI H Z, et al. STAT3 mutations in the hyper-IgE syndrome ［J］. New Eng J Med, 2007, 357(16):1608-1619.

［15］LITONJUA A A, TANTISIRA K G, LAKE S, et al. Polymorphisms in signal transducer and activator of transcription 3 and lung function in asthma ［J］. Respir Res, 2005, 6: 52.

［16］ZHONG Y, FENG J, CHEN B, et al. Signal transducer and activator of transcription 3 (STAT3) gene polymorphisms are associated with treatment outcomes in acute myeloid leukemia ［J］. Int J Lab Hematol, 2012, 34(4):383-389.

［17］NAIR R R, TOLENTINO J H, HAZLEHURST L A. Role of STAT3 in Transformation and Drug Resistance in CML ［J］. Front Oncol, 2012, 2: 30.

［18］TAMURA G, YIN J, WANG S, et al. E-Cadherin gene promoter hypermethylation in primary human gastric carcinomas ［J］. J Natl Cancer Ins, 2000, 92(7):569-573.

［19］DARNELL JR J E. STATs and gene regulation ［J］. Science, 1997, 277(5332):1630-1635.

［20］KIU H, NICHOLSON S. Biology and significance of the JAK/STAT signalling pathways ［J］. Growth Factors, 2012, 30(2):88-106.

［21］SEITA J, ASAKAWA M, OOEHARA J, et al. Interleukin-27 directly induces differentiation in hematopoietic stem cells ［J］. Blood, 2008, 111(4):1903-1912.

［22］HANKEY P. Regulation of hematopoietic cell development and function by Stat3 ［J］. Front Biosci, 2009, 14(1):5273-5290.

［23］KHATIB H, HUANG W, MIKHEIL D, et al. Effects of signal transducer and activator of transcription (STAT) genes STAT1 and STAT3 genotypic combinations on fertilization and embryonic survival rates in Holstein cattle ［J］. J Dairy Sci, 2009, 92(12):6186-6191.

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Associations of Polymorphisms of Promoter Region of STAT3 Gene with Growth Traits in Wuchuan Black Cattle

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